Part:BBa_J70210:Design

A BioScaffold Part (Uses PpiI), similar to J70010 see Part Design Page

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 25
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 49
  Illegal BsaI.rc site found at 1
  

Design Notes

Same as J70010 except uses AscI instead of PacI

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Can be used to cut at 0 offset from the BioBrick scar on either side, see BBF RFC15

TACTAGAG and TACAGAT represent sequences for BioBrick scars that are created when

two BioBrick parts are fused together.

^s represent restriction enzyme cut sites.

General Function

XXXXX XXXXX^TACTAGAG BioScaffold Part BBa_J70010 TACTAGAT XXXXX^XXXXX

XXXXX^XXXXX ATGATCAC ATGATCTA^XXXXX XXXXX

Function when the right part is a protein part.

XXXXX XXXXX^TACTAGAG BioScaffold Part BBa_J70010 TACTAGAT GXXXX^XXXXX

XXXXX^XXXXX ATGATCAC ATGATCTA^CXXXX XXXXX

What happens after cutting with MabI or PacI and purifying with either gel purification

or a Qiagen PCR cleanup spin column

General Case

XXXXX XXXXX^ ^XXXXX

XXXXX^ ^XXXXX XXXXX

Case when right part is a protein part

XXXXX XXXXX^ ^XXXXX

XXXXX^ ^CXXXX XXXXX

Source

designed